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A Method for Enzymic Extraction and the Measurement of Chloroplast RNA

A method is described for measuring RNA associated with chloroplast thylakoid membranes. Washed thylakoids are incubated with ribonucleases A and T(1), under low Mg(2+) conditions, to release hydrolyzed RNA into solution. After removing the membranes by centrifugation, the mono- and oligonucleotides...

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Detalhes bibliográficos
Main Authors: Fish, Leonard, Jagendorf, André T.
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC440415/
https://ncbi.nlm.nih.gov/pubmed/16661271
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