Targeted construction of temperature-sensitive mutations in vaccinia virus by replacing clustered charged residues with alanine.
The feasibility of using "clustered charge-to-alanine" mutagenesis (replacement by alanine of two or more charged residues clustered in a five- or six-amino acid sequence) to create temperature-sensitive, conditionally lethal mutations in vaccinia virus was examined by creating nine mutations in the...
Gardado en:
| Publicado en: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1994
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC43824/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8183946/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.10.4554 |
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