Clustered Charge-to-Alanine Mutagenesis of the Vaccinia Virus A20 Gene: Temperature-Sensitive Mutants Have a DNA-Minus Phenotype and Are Defective in the Production of Processive DNA Polymerase Activity

Although the vaccinia virus DNA polymerase is inherently distributive, a highly processive form of the enzyme exists within the cytoplasm of infected cells (W. F. McDonald, N. Klemperer, and P. Traktman, Virology 234:168–175, 1997). In the accompanying report we outline the purification of the 49-kD...

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Detalhes bibliográficos
Main Authors: Punjabi, Almira, Boyle, Kathleen, DeMasi, Joseph, Grubisha, Olivera, Unger, Beth, Khanna, Marilyn, Traktman, Paula
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Replication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC116127/
https://ncbi.nlm.nih.gov/pubmed/11711621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.75.24.12308-12318.2001
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