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Asymmetric mutations in the tetrameric R67 dihydrofolate reductase reveal high tolerance to active-site substitutions

Type II R67 dihydrofolate reductase (DHFR) is a bacterial plasmid-encoded enzyme that is intrinsically resistant to the widely-administered antibiotic trimethoprim. R67 DHFR is genetically and structurally unrelated to E. coli chromosomal DHFR and has an unusual architecture, in that four identical...

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Publicat a:Protein Sci
Autors principals: Ebert, Maximilian C C J C, Morley, Krista L, Volpato, Jordan P, Schmitzer, Andreea R, Pelletier, Joelle N
Format: Artigo
Idioma:Inglês
Publicat: Blackwell Publishing Ltd 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4380981/
https://ncbi.nlm.nih.gov/pubmed/25401264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2602
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