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A CRISPR/Cas9 vector system for tissue-specific gene disruption in zebrafish
CRISPR/Cas9 technology of genome editing has greatly facilitated the targeted inactivation of genes in vitro and in vivo in a wide range of organisms. In zebrafish it allows the rapid generation of knock-out lines by simply injecting a guide RNA (gRNA) and Cas9 mRNA into one-cell stage embryos. Here...
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| Publicado no: | Dev Cell |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4379706/ https://ncbi.nlm.nih.gov/pubmed/25752963 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.devcel.2015.01.032 |
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