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Upgraded CRISPR/Cas9 tools for tissue-specific mutagenesis in Drosophila
CRISPR/Cas9 has emerged as a powerful technology for tissue-specific mutagenesis. However, tissue-specific CRISPR/Cas9 tools currently available in Drosophila remain deficient in three significant ways. First, many existing gRNAs are inefficient, such that further improvements of gRNA expression con...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
2021
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8040800/ https://ncbi.nlm.nih.gov/pubmed/33782117 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2014255118 |
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