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Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
Here, we present a simple and highly efficient method for generating and detecting mutations of any gene in Drosophila melanogaster through the use of the CRISPR/Cas9 system (clustered regularly interspaced palindromic repeats/CRISPR-associated). We show that injection of RNA into the Drosophila emb...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cell Press
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3714591/ https://ncbi.nlm.nih.gov/pubmed/23827738 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2013.06.020 |
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