Tyrosyl-DNA Phosphodiesterase I Catalytic Mutants Reveal an Alternative Nucleophile That Can Catalyze Substrate Cleavage

Tyrosyl-DNA phosphodiesterase I (Tdp1) catalyzes the repair of 3′-DNA adducts, such as the 3′-phosphotyrosyl linkage of DNA topoisomerase I to DNA. Tdp1 contains two conserved catalytic histidines: a nucleophilic His (His(nuc)) that attacks DNA adducts to form a covalent 3′-phosphohistidyl intermedi...

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Bibliographic Details
Published in:J Biol Chem
Main Authors: Comeaux, Evan Q., Cuya, Selma M., Kojima, Kyoko, Jafari, Nauzanene, Wanzeck, Keith C., Mobley, James A., Bjornsti, Mary-Ann, van Waardenburg, Robert C. A. M.
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2015
Subjects:
DNA and Chromosomes
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4358259/
https://ncbi.nlm.nih.gov/pubmed/25609251
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.635284
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