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Isolation of Myofibroblasts from Mouse and Human Esophagus

Murine and human esophageal myofibroblasts are generated via enzymatic digestion. Neonate (8-12 day old) murine esophagus is harvested, minced, washed, and subjected to enzymatic digestion with collagenase and dispase for 25 min. Human esophageal resection specimens are stripped of muscularis propri...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Vis Exp
Prif Awduron: Gargus, Matthew, Niu, Chao, Shaker, Anisa
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MyJove Corporation 2015
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354535/
https://ncbi.nlm.nih.gov/pubmed/25650889
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52215
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