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Isolation of Myofibroblasts from Mouse and Human Esophagus
Murine and human esophageal myofibroblasts are generated via enzymatic digestion. Neonate (8-12 day old) murine esophagus is harvested, minced, washed, and subjected to enzymatic digestion with collagenase and dispase for 25 min. Human esophageal resection specimens are stripped of muscularis propri...
Salvato in:
| Pubblicato in: | J Vis Exp |
|---|---|
| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
MyJove Corporation
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4354535/ https://ncbi.nlm.nih.gov/pubmed/25650889 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52215 |
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