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Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens

Fibroblasts/myofibroblasts (MFs) have been gaining increasing attention for their role in pathogenesis and their contributions to both wound healing and promotion of the tumor microenvironment. While there are currently many techniques for the isolation of MFs from gastrointestinal (GI) tissues, thi...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Johnson, Paul, Beswick, Ellen J., Chao, Celia, Powell, Don W., Hellmich, Mark R., Pinchuk, Iryna V.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4781712/
https://ncbi.nlm.nih.gov/pubmed/26863470
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53691
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