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Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
The purpose of this protocol is to fractionate human intestinal tissue obtained by endoscopy into nuclear and cytoplasmic compartments for the localization analysis of specific proteins or protein complexes in different tissue states (i.e., healthy vs. disease). This method is useful for the fractio...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6126469/ https://ncbi.nlm.nih.gov/pubmed/30059028 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57740 |
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