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Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
The purpose of this protocol is to fractionate human intestinal tissue obtained by endoscopy into nuclear and cytoplasmic compartments for the localization analysis of specific proteins or protein complexes in different tissue states (i.e., healthy vs. disease). This method is useful for the fractio...
Uloženo v:
| Vydáno v: | J Vis Exp |
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| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
MyJove Corporation
2018
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6126469/ https://ncbi.nlm.nih.gov/pubmed/30059028 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57740 |
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