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Isolation of Myofibroblasts from Mouse and Human Esophagus
Murine and human esophageal myofibroblasts are generated via enzymatic digestion. Neonate (8-12 day old) murine esophagus is harvested, minced, washed, and subjected to enzymatic digestion with collagenase and dispase for 25 min. Human esophageal resection specimens are stripped of muscularis propri...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4354535/ https://ncbi.nlm.nih.gov/pubmed/25650889 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52215 |
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