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Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Protein degradation by the ubiquitin-proteasome system (UPS) is a major regulatory mechanism for protein homeostasis in all eukaryotes. The standard approach to determining intracellular protein degradation relies on biochemical assays for following the kinetics of protein decline. Such methods are...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Cohen, Itamar, Geffen, Yifat, Ravid, Guy, Ravid, Tommer
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4353405/
https://ncbi.nlm.nih.gov/pubmed/25406949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52021
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