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Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
Protein degradation by the ubiquitin-proteasome system (UPS) is a major regulatory mechanism for protein homeostasis in all eukaryotes. The standard approach to determining intracellular protein degradation relies on biochemical assays for following the kinetics of protein decline. Such methods are...
Gardado en:
| Publicado en: | J Vis Exp |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2014
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4353405/ https://ncbi.nlm.nih.gov/pubmed/25406949 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52021 |
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