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Comparison of T7E1 and Surveyor Mismatch Cleavage Assays to Detect Mutations Triggered by Engineered Nucleases

Genome editing using engineered nucleases is used for targeted mutagenesis. But because genome editing does not target all loci with similar efficiencies, the mutation hit-rate at a given locus needs to be evaluated. The analysis of mutants obtained using engineered nucleases requires specific metho...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
में प्रकाशित:G3 (Bethesda)
मुख्य लेखकों: Vouillot, Léna, Thélie, Aurore, Pollet, Nicolas
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: Genetics Society of America 2015
विषय:
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC4349094/
https://ncbi.nlm.nih.gov/pubmed/25566793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.114.015834
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