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Double replacement: strategy for efficient introduction of subtle mutations into the murine Col1a-1 gene by homologous recombination in embryonic stem cells.

A subtle mutation that rendered type I collagen resistant to mammalian collagenase has been introduced into the murine Col1a-1 (recently redesignated Cola-1) gene by homologous recombination in embryonic stem (ES) cells. Initially, a "hit and run" procedure was used. Since two steps were r...

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Hlavní autoři: Wu, H, Liu, X, Jaenisch, R
Médium: Artigo
Jazyk:Inglês
Vydáno: 1994
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC43462/
https://ncbi.nlm.nih.gov/pubmed/8146196
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