Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules

We systematically varied conditions of two-dimensional (2D) agarose gel electrophoresis to optimize separation of DNA topoisomers that differ either by the extent of knotting, the extent of catenation or the extent of supercoiling. To this aim we compared electrophoretic behavior of three different...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Autores principales: Cebrián, Jorge, Kadomatsu-Hermosa, Maridian J., Castán, Alicia, Martínez, Víctor, Parra, Cristina, Fernández-Nestosa, María José, Schaerer, Christian, Martínez-Robles, María-Luisa, Hernández, Pablo, Krimer, Dora B., Stasiak, Andrzej, Schvartzman, Jorge B.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4344484/
https://ncbi.nlm.nih.gov/pubmed/25414338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku1255
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