Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules

We systematically varied conditions of two-dimensional (2D) agarose gel electrophoresis to optimize separation of DNA topoisomers that differ either by the extent of knotting, the extent of catenation or the extent of supercoiling. To this aim we compared electrophoretic behavior of three different...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Cebrián, Jorge, Kadomatsu-Hermosa, Maridian J., Castán, Alicia, Martínez, Víctor, Parra, Cristina, Fernández-Nestosa, María José, Schaerer, Christian, Martínez-Robles, María-Luisa, Hernández, Pablo, Krimer, Dora B., Stasiak, Andrzej, Schvartzman, Jorge B.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2015
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4344484/
https://ncbi.nlm.nih.gov/pubmed/25414338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku1255
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