A general method to improve fluorophores for live-cell and single-molecule microscopy

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes inside living cells, resulting in brighter reporters than fluorescent proteins. Intracellular labeling using these te...

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Bibliographic Details
Published in:Nat Methods
Main Authors: Grimm, Jonathan B., English, Brian P., Chen, Jiji, Slaughter, Joel P., Zhang, Zhengjian, Revyakin, Andrey, Patel, Ronak, Macklin, John J., Normanno, Davide, Singer, Robert H., Lionnet, Timothée, Lavis, Luke D.
Format: Artigo
Language:Inglês
Published: 2015
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4344395/
https://ncbi.nlm.nih.gov/pubmed/25599551
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3256
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