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Quantitative real-time kinetics of optogenetic proteins CRY2 and CIB1/N using single-molecule tools

In this work we evaluate the interaction of two optogenetic protein variants (CIB1, CIBN) with their complementary protein CRY2 by single-molecule tools in cell-free extracts. After validating the blue light induced co-localization of CRY2 and CIB1/N by Förster resonance energy transfer (FRET) in li...

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Publicat a:Anal Biochem
Autors principals: Cui, Yi, Choudhury, Samrat Roy, Irudayaraj, Joseph
Format: Artigo
Idioma:Inglês
Publicat: 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4341968/
https://ncbi.nlm.nih.gov/pubmed/24780222
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.04.023
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