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Quantitative real-time kinetics of optogenetic proteins CRY2 and CIB1/N using single-molecule tools
In this work we evaluate the interaction of two optogenetic protein variants (CIB1, CIBN) with their complementary protein CRY2 by single-molecule tools in cell-free extracts. After validating the blue light induced co-localization of CRY2 and CIB1/N by Förster resonance energy transfer (FRET) in li...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4341968/ https://ncbi.nlm.nih.gov/pubmed/24780222 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.04.023 |
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