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Quantitative Transcription Factor Binding Kinetics at the Single-Molecule Level
We investigated the binding interaction between the bacteriophage λ-repressor CI and its target DNA using total internal reflection fluorescence microscopy. Large stepwise changes in the intensity of the red fluorescent protein fused to CI were observed as it associated with and dissociated from ind...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2716481/ https://ncbi.nlm.nih.gov/pubmed/19167308 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.09.040 |
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