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A Single-Molecule Technique to Study Sequence-Dependent Transcription Pausing
We present a technique that allows sequence-dependent analysis of transcription elongation using single-molecule optical trapping techniques. Observation of individual molecules of RNA polymerase (RNAP) allows determination of elongation kinetics that are difficult or impossible to accurately obtain...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Biophysical Society
2004
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1304905/ https://ncbi.nlm.nih.gov/pubmed/15465875 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.044081 |
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