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A Single-Molecule Technique to Study Sequence-Dependent Transcription Pausing

We present a technique that allows sequence-dependent analysis of transcription elongation using single-molecule optical trapping techniques. Observation of individual molecules of RNA polymerase (RNAP) allows determination of elongation kinetics that are difficult or impossible to accurately obtain...

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Bibliografiska uppgifter
Huvudupphovsmän: Shundrovsky, Alla, Santangelo, Thomas J., Roberts, Jeffrey W., Wang, Michelle D.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Biophysical Society 2004
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304905/
https://ncbi.nlm.nih.gov/pubmed/15465875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.044081
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