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A Single-Molecule Technique to Study Sequence-Dependent Transcription Pausing

We present a technique that allows sequence-dependent analysis of transcription elongation using single-molecule optical trapping techniques. Observation of individual molecules of RNA polymerase (RNAP) allows determination of elongation kinetics that are difficult or impossible to accurately obtain...

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Autors principals: Shundrovsky, Alla, Santangelo, Thomas J., Roberts, Jeffrey W., Wang, Michelle D.
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304905/
https://ncbi.nlm.nih.gov/pubmed/15465875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.044081
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