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Sequence-Dependent Pausing of Single Lambda Exonuclease Molecules

Lambda exonuclease processively degrades one strand of duplex DNA, moving 5′-to-3′ in an ATP-independent fashion. When examined at the single-molecule level, the speeds of digestion were nearly constant at 4 nanometers per second (12 nucleotides per second), interspersed with pauses of variable dura...

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Bibliografiske detaljer
Main Authors: Perkins, Thomas T., Dalal, Ravindra V., Mitsis, Paul G., Block, Steven M.
Format: Artigo
Sprog:Inglês
Udgivet: 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1539570/
https://ncbi.nlm.nih.gov/pubmed/12947034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1088047
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