Forward and Reverse Motion of Single RecBCD Molecules on DNA

RecBCD is a processive, DNA-based motor enzyme with both helicase and nuclease activities. We used high-resolution optical trapping to study individual RecBCD molecules moving against applied forces up to 8 pN. Fine-scale motion was smooth down to a detection limit of 2 nm, implying a unitary step s...

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Библиографические подробности
Главные авторы: Perkins, Thomas T., Li, Hung-Wen, Dalal, Ravindra V., Gelles, Jeff, Block, Steven M.
Формат: Artigo
Язык:Inglês
Опубликовано: Biophysical Society 2004
Предметы:
Nucleic Acids
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC1303999/
https://ncbi.nlm.nih.gov/pubmed/14990491
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