Sequence-dependent nanometer-scale conformational dynamics of individual RecBCD–DNA complexes

RecBCD is a multifunctional enzyme that possesses both helicase and nuclease activities. To gain insight into the mechanism of its helicase function, RecBCD unwinding at low adenosine triphosphate (ATP) (2–4 μM) was measured using an optical-trapping assay featuring 1 base-pair (bp) precision. Inste...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Carter, Ashley R., Seaberg, Maasa H., Fan, Hsiu-Fang, Sun, Gang, Wilds, Christopher J., Li, Hung-Wen, Perkins, Thomas T.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2016
Assuntos:
Nucleic Acid Enzymes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4937329/
https://ncbi.nlm.nih.gov/pubmed/27220465
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw445
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