Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in electrophoretic mobility shift assays

BACKGROUND: Highly purified nuclear protein is required when using an electrophoretic mobility shift assay (EMSA) to study transcription factors, e.g. nuclear factor-κB (NF-κB), a major transcription factor that regulates both innate and adaptive immune responses following infection. Although many p...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:BMC Immunol
Prif Awduron: Luo, Yuqian, Hara, Takeshi, Ishido, Yuko, Yoshihara, Aya, Oda, Kenzaburo, Makino, Masahiko, Ishii, Norihisa, Hiroi, Naoki, Suzuki, Koichi
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: BioMed Central 2014
Pynciau:
Methodology Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4339431/
https://ncbi.nlm.nih.gov/pubmed/25527077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12865-014-0062-z
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