Purification and Separation of Pyridine Nucleotide-Linked Dehydrogenases by Affinity Chromatography Techniques

A number of different dehydrogenases have been shown to bind to Sepharose-bound N(6)-(6-aminohexyl)-AMP. These dehydrogenases can be specifically eluted by binary adducts of NAD(+) or with cofactor gradients. In such manner pure enzymes can be obtained from crude extracts, as demonstrated in the pur...

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Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Main Authors: Kaplan, Nathan O., Everse, Johannes, Dixon, Jack E., Stolzenbach, Francis E., Lee, Chi-Yu, Lee, Ching-Lun T., Taylor, Susan S., Mosbach, Klaus
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1974
Teme:
Biological Sciences: Biochemistry
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433791/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4372619/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.9.3450
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