Purification and Separation of Pyridine Nucleotide-Linked Dehydrogenases by Affinity Chromatography Techniques

A number of different dehydrogenases have been shown to bind to Sepharose-bound N(6)-(6-aminohexyl)-AMP. These dehydrogenases can be specifically eluted by binary adducts of NAD(+) or with cofactor gradients. In such manner pure enzymes can be obtained from crude extracts, as demonstrated in the pur...

Description complète

Détails bibliographiques

Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Kaplan, Nathan O., Everse, Johannes, Dixon, Jack E., Stolzenbach, Francis E., Lee, Chi-Yu, Lee, Ching-Lun T., Taylor, Susan S., Mosbach, Klaus
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 1974
Sujets:	Biological Sciences: Biochemistry
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433791/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4372619/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.9.3450
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!