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Combined use of strain construction and affinity chromatography in the rapid, high-yield purification of 6-phosphogluconate dehydrogenase from Escherichia coli.

A rapid, high-yield method for purification of 6-phosphogluconate dehydrogenase from Escherichia coli K-12 is described. Sonic extracts prepared from heat-induced cultures of strain RW184, doubly lysogenic for the specialized transducing bacteriophage lambdacI857St68h80dgndhis and bearing a deletion...

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Détails bibliographiques
Auteurs principaux: Wolf, R E, Shea, F M
Format: Artigo
Langue:Inglês
Publié: 1979
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC218254/
https://ncbi.nlm.nih.gov/pubmed/35519
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