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Single-Molecule Imaging at High Fluorophore Concentrations by Local Activation of Dye

Single-molecule fluorescence microscopy is a powerful tool for observing biomolecular interactions with high spatial and temporal resolution. Detecting fluorescent signals from individual labeled proteins above high levels of background fluorescence remains challenging, however. For this reason, the...

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書目詳細資料
發表在:Biophys J
Main Authors: Geertsema, Hylkje J., Schulte, Aartje C., Spenkelink, Lisanne M., McGrath, William J., Morrone, Seamus R., Sohn, Jungsan, Mangel, Walter F., Robinson, Andrew, van Oijen, Antoine M.
格式: Artigo
語言:Inglês
出版: The Biophysical Society 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4336379/
https://ncbi.nlm.nih.gov/pubmed/25692599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.12.019
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