Single-Molecule Imaging at High Fluorophore Concentrations by Local Activation of Dye

Single-molecule fluorescence microscopy is a powerful tool for observing biomolecular interactions with high spatial and temporal resolution. Detecting fluorescent signals from individual labeled proteins above high levels of background fluorescence remains challenging, however. For this reason, the...

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Detalles Bibliográficos
Publicado en:Biophys J
Autores principales: Geertsema, Hylkje J., Schulte, Aartje C., Spenkelink, Lisanne M., McGrath, William J., Morrone, Seamus R., Sohn, Jungsan, Mangel, Walter F., Robinson, Andrew, van Oijen, Antoine M.
Formato: Artigo
Lenguaje:Inglês
Publicado: The Biophysical Society 2015
Materias:
Proteins and Nucleic Acids
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4336379/
https://ncbi.nlm.nih.gov/pubmed/25692599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.12.019
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