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Single-Molecule Imaging at High Fluorophore Concentrations by Local Activation of Dye
Single-molecule fluorescence microscopy is a powerful tool for observing biomolecular interactions with high spatial and temporal resolution. Detecting fluorescent signals from individual labeled proteins above high levels of background fluorescence remains challenging, however. For this reason, the...
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Publicado no: | Biophys J |
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Main Authors: | , , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
The Biophysical Society
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4336379/ https://ncbi.nlm.nih.gov/pubmed/25692599 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.12.019 |
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