Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

Pichia pastoris is a prominent host for recombinant protein production, amongst other things due to its capability of glycosylation. However, N-linked glycans on recombinant proteins get hypermannosylated, causing problems in subsequent unit operations and medical applications. Hypermannosylation is...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Gmeiner, Christoph, Saadati, Amirhossein, Maresch, Daniel, Krasteva, Stanimira, Frank, Manuela, Altmann, Friedrich, Herwig, Christoph, Spadiut, Oliver
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4335410/
https://ncbi.nlm.nih.gov/pubmed/25567661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-014-0183-3
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