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Current Protocols in Protein Science: Analysis of protein stability and ligand interactions by thermal shift assay

The purification of recombinant proteins for biochemical assays and structural studies is time-consuming and presents inherent difficulties that depend on the optimization of protein stability. The use of dyes to monitor thermal denaturation of proteins with sensitive fluorescence detection enables...

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Detalhes bibliográficos
Publicado no:Curr Protoc Protein Sci
Main Authors: Huynh, Kathy, Partch, Carrie L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4332540/
https://ncbi.nlm.nih.gov/pubmed/25640896
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps2809s79
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