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Current Protocols in Protein Science: Analysis of protein stability and ligand interactions by thermal shift assay
The purification of recombinant proteins for biochemical assays and structural studies is time-consuming and presents inherent difficulties that depend on the optimization of protein stability. The use of dyes to monitor thermal denaturation of proteins with sensitive fluorescence detection enables...
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| Veröffentlicht in: | Curr Protoc Protein Sci |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4332540/ https://ncbi.nlm.nih.gov/pubmed/25640896 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps2809s79 |
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