Llwytho...

Ligand induced stabilization of the melting temperature of the HSV-1 single-strand DNA binding protein using the thermal shift assay

We have adapted the thermal shift assay to measure the ligand binding properties of the herpes simplex virus-1 single-strand DNA binding protein, ICP8. By measuring SYPRO Orange fluorescence in microtiter plates using a fluorescence-enabled thermal cycler, we have quantified the effects of oligonucl...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Biochem Biophys Res Commun
Prif Awduron: Rupesh, Kanchi Ravi, Smith, Aaron, Boehmer, Paul E.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2014
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4254511/
https://ncbi.nlm.nih.gov/pubmed/25449284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2014.10.145
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