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High-throughput detection of DNA double-strand breaks using image cytometry

Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting...

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Publicat a:Biotechniques
Autors principals: Fowler, Tyler L., Bailey, Alison M., Bednarz, Bryan P., Kimple, Randall J.
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4331074/
https://ncbi.nlm.nih.gov/pubmed/25605579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114248
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