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High-throughput quantitative detection of basal autophagy and autophagic flux using image cytometry
Quantitative assessment of changes in macro-autophagy is often performed through manual quantification of the number of LC3B foci in immunofluorescence microscopy images. This method is highly laborious, subject to image-field selection and foci-counting bias, and is not sensitive for analyzing chan...
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| Pubblicato in: | Biotechniques |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7141596/ https://ncbi.nlm.nih.gov/pubmed/31238709 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2019-0044 |
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