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High-throughput quantitative detection of basal autophagy and autophagic flux using image cytometry

Quantitative assessment of changes in macro-autophagy is often performed through manual quantification of the number of LC3B foci in immunofluorescence microscopy images. This method is highly laborious, subject to image-field selection and foci-counting bias, and is not sensitive for analyzing chan...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:Biotechniques
Asıl Yazarlar: SenthilKumar, Gopika, Skiba, Justin H., Kimple, Randall J.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2019
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7141596/
https://ncbi.nlm.nih.gov/pubmed/31238709
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2019-0044
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