Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues

RNA sequencing measures the quantitative change in gene expression over the whole transcriptome, but it lacks spatial context. On the other hand, in situ hybridization provides the location of gene expression, but only for a small number of genes. Here we detail a protocol for genome-wide profiling...

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發表在:Nat Protoc
Main Authors: Lee, Je Hyuk, Daugharthy, Evan R., Scheiman, Jonathan, Kalhor, Reza, Ferrante, Thomas C., Terry, Richard, Turczyk, Brian M., Yang, Joyce L., Lee, Ho Suk, Aach, John, Zhang, Kun, Church, George M.
格式: Artigo
語言:Inglês
出版: 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4327781/
https://ncbi.nlm.nih.gov/pubmed/25675209
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.191
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