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Accelerating Genome Editing in CHO Cells Using CRISPR Cas9 and CRISPy, a Web-Based Target Finding Tool

Chinese hamster ovary (CHO) cells are widely used in the biopharmaceutical industry as a host for the production of complex pharmaceutical proteins. Thus genome engineering of CHO cells for improved product quality and yield is of great interest. Here, we demonstrate for the first time the efficacy...

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Detalhes bibliográficos
Publicado no:Biotechnol Bioeng
Main Authors: Ronda, Carlotta, Pedersen, Lasse Ebdrup, Hansen, Henning Gram, Kallehauge, Thomas Beuchert, Betenbaugh, Michael J, Nielsen, Alex Toftgaard, Kildegaard, Helene Faustrup
Formato: Artigo
Idioma:Inglês
Publicado em: BlackWell Publishing Ltd 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4312910/
https://ncbi.nlm.nih.gov/pubmed/24827782
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.25233
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