Loading...
A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase
[Image: see text] Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without...
Saved in:
| Published in: | Biochemistry |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American
Chemical Society
2014
|
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4310628/ https://ncbi.nlm.nih.gov/pubmed/25537790 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi501198f |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|