A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase

[Image: see text] Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without...

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Bibliographic Details
Published in:Biochemistry
Main Authors: Wu, Eugene Y., Walsh, Amanda R., Materne, Emma C., Hiltner, Emily P., Zielinski, Bryan, Miller, Bill R., Mawby, Lily, Modeste, Erica, Parish, Carol A., Barnes, Wayne M., Kermekchiev, Milko B.
Format: Artigo
Language:Inglês
Published: American Chemical Society 2014
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4310628/
https://ncbi.nlm.nih.gov/pubmed/25537790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi501198f
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