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A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase
[Image: see text] Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without...
Zapisane w:
| Wydane w: | Biochemistry |
|---|---|
| Główni autorzy: | , , , , , , , , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American
Chemical Society
2014
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| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4310628/ https://ncbi.nlm.nih.gov/pubmed/25537790 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi501198f |
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