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Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR
Although the thermophilic bacterium Thermus aquaticus grows optimally at 70°C and cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20–37°C. This activity is a bane to some PCRs, since it catalyzes non-specific priming. We report mutations of Klentaq (an N-termin...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2003
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC275455/ https://ncbi.nlm.nih.gov/pubmed/14576300 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkg813 |
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