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Direct DNA Amplification from Crude Clinical Samples Using a PCR Enhancer Cocktail and Novel Mutants of Taq

PCR-based clinical and forensic tests often have low sensitivity or even false-negative results caused by potent PCR inhibitors found in blood and soil. It is widely accepted that purification of target DNA before PCR is necessary for successful amplification. In an attempt to overcome PCR inhibitio...

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Détails bibliographiques
Auteurs principaux: Zhang, Zhian, Kermekchiev, Milko B., Barnes, Wayne M.
Format: Artigo
Langue:Inglês
Publié: American Society for Investigative Pathology 2010
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2871721/
https://ncbi.nlm.nih.gov/pubmed/20075207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2353/jmoldx.2010.090070
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