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Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing

The copper(II) centre of the blue copper protein pseudoazurin from Alcaligenes faecalis has been substituted by zinc(II) via denaturing the protein, chelation and removal of copper and refolding the apoprotein, followed by the addition of an aqueous solution of ZnCl(2). Vapour-diffusion experiments...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr F Struct Biol Commun
Main Authors: Gessmann, Renate, Papadovasilaki, Maria, Drougkas, Evangelos, Petratos, Kyriacos
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4304741/
https://ncbi.nlm.nih.gov/pubmed/25615962
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14025552
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