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Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing
The copper(II) centre of the blue copper protein pseudoazurin from Alcaligenes faecalis has been substituted by zinc(II) via denaturing the protein, chelation and removal of copper and refolding the apoprotein, followed by the addition of an aqueous solution of ZnCl(2). Vapour-diffusion experiments...
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| Publicado en: | Acta Crystallogr F Struct Biol Commun |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
International Union of Crystallography
2015
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4304741/ https://ncbi.nlm.nih.gov/pubmed/25615962 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14025552 |
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