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Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing
The copper(II) centre of the blue copper protein pseudoazurin from Alcaligenes faecalis has been substituted by zinc(II) via denaturing the protein, chelation and removal of copper and refolding the apoprotein, followed by the addition of an aqueous solution of ZnCl(2). Vapour-diffusion experiments...
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| Udgivet i: | Acta Crystallogr F Struct Biol Commun |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
International Union of Crystallography
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4304741/ https://ncbi.nlm.nih.gov/pubmed/25615962 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14025552 |
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