Biophysical and enzymatic properties of aminoglycoside adenylyltransferase AadA6 from Pseudomonas aeruginosa

The gene coding for the aminoglycoside adenylyltransferase (aadA6) from a clinical isolate of Pseudomonas aeruginosa was cloned and expressed in Escherichia coli strain BL21(DE3)pLysS. The overexpressed enzyme (AadA6, 281 amino-acid residues) and a carboxy-terminal truncated variant molecule ([1-264...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Rep
Main Authors: Papadovasilaki, Maria, Oberthür, Dominik, Gessmann, Renate, Sarrou, Iosifina, Betzel, Christian, Scoulica, Effie, Petratos, Kyriacos
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5668923/
https://ncbi.nlm.nih.gov/pubmed/29124199
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2015.09.011
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