A general protocol for the generation of Nanobodies for structural biology

There is growing interest in using antibodies as auxiliary proteins to crystallize proteins. Here, we describe a general protocol for the generation of Nanobodies to be used as crystallization chaperones for the structural investigation of diverse conformational states of flexible (membrane) protein...

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Publicado en:Nat Protoc
Autores principales: Pardon, Els, Laeremans, Toon, Triest, Sarah, Rasmussen, Søren G. F., Wohlkönig, Alexandre, Ruf, Armin, Muyldermans, Serge, Hol, Wim G. J., Kobilka, Brian K., Steyaert, Jan
Formato: Artigo
Lenguaje:Inglês
Publicado: 2014
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4297639/
https://ncbi.nlm.nih.gov/pubmed/24577359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.039
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